J. Sowell et al., Capillary electrophoresis-based immunoassay for insulin antibodies with near-infrared laser induced fluorescence detection, J CHROMAT B, 752(1), 2001, pp. 1-8
ii noncompetitive capillary electrophoresis (CE)-based immunoassay with nea
r-infrared laser induced fluorescence detection (NIR-LIF) for insulin antib
odies has been developed. In the assay, insulin was derivatized with a NIR
fluorescent dye (NN382; LI-COR). Insulin antibodies were detected via the f
ormation of an immunocomplex. Parameters affecting the separation such as p
H, voltage and ionic strength were investigated. Furthermore, it was found
that increasing the ramp time of the applied voltage improved the detection
limit of the assay by an order of magnitude. The detection limit of the as
say was 1.1 nM. (C) 2001 Elsevier Science B.V. All rights reserved.