Capillary electrophoresis-based immunoassay for insulin antibodies with near-infrared laser induced fluorescence detection

ii noncompetitive capillary electrophoresis (CE)-based immunoassay with nea r-infrared laser induced fluorescence detection (NIR-LIF) for insulin antib odies has been developed. In the assay, insulin was derivatized with a NIR fluorescent dye (NN382; LI-COR). Insulin antibodies were detected via the f ormation of an immunocomplex. Parameters affecting the separation such as p H, voltage and ionic strength were investigated. Furthermore, it was found that increasing the ramp time of the applied voltage improved the detection limit of the assay by an order of magnitude. The detection limit of the as say was 1.1 nM. (C) 2001 Elsevier Science B.V. All rights reserved.