Development and validation of a column-switching high-performance liquid chromatographic method for the determination of sanfetrinem in rat and dog plasma by direct injection

A direct injection column-switching HPLC method was developed and validated for quantification of sanfetrinem in rat and dog plasma. Following dilutio n with buffer, samples were directly injected onto the system. The analyte was retained in an enrichment column while endogenous plasma components wer e eluted to waste. Sanfetrinem was then back-flushed to the analytical colu mn for separation and quantification with an ultraviolet detector. Sample b atch size was increased by adding a washing phase of the enrichment column and by alternating the injections between two enrichment columns. The metho d is very simple and sample preparation is minimal. The method has been ful ly Validated and shown to be specific, accurate and reproducible. (C) 2001 Elsevier Science B.V. All rights reserved.