G. Pistritto et al., Signaling pathways involved in lipopolysaccharide stimulation of prostaglandin production by rat hypothalamic astroglial cells, J ENDOTOX R, 6(4), 2000, pp. 307-311
The aim of this study was to investigate the mechanisms through which bacte
rial lipopolysaccharide (LPS) stimulates prostaglandin (PG) production in r
at hypothalamic astroglial cells in vitro. The latter were treated with LPS
alone or LPS plus antagonists of the interleukin-1 (IL-1) and nitric oxide
(NO) pathways, and the subsequent changes in cyclooxygenase (COX) activity
were monitored by measuring a COX end-product, prostaglandin E-2 (PGE(2)),
released into the incubation medium. LPS produced a concentration-dependen
t increase in PGE, release from astroglia after 24 h incubation; experiment
s with selective antagonists showed that the increase in PGE(2) release ind
uced by LPS may be, at least in part, mediated by IL-1 and NO.