Enhanced production of vascular endothelial growth factor by human monocytic cells stimulated with endotoxin through transcription factor SP-1

Authors
Sakuta, T Matsushita, K Yamaguchi, N Oyama, T Motani, R Koga, T Nagaoka, S Abeyama, K Maruyama, K Takada, H Torii, M
Citation
T. Sakuta et al., Enhanced production of vascular endothelial growth factor by human monocytic cells stimulated with endotoxin through transcription factor SP-1, J MED MICRO, 50(3), 2001, pp. 233-237
Citations number
21
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF MEDICAL MICROBIOLOGY
ISSN journal
00222615 → ACNP
Volume
50
Issue
3
Year of publication
2001
Pages
233 - 237
Database
ISI
SICI code
0022-2615(200103)50:3<233:EPOVEG>2.0.ZU;2-J
Abstract
The effect of endotoxin on the regulation of vascular endothelial growth fa ctor (VEGF) mRNA expression in human monocytic (THP-1) cells was examined. Endotoxic lipopolysaccharide (LPS) from Escherichia coli and synthetic E, c oli-type lipid A (LA-15-PP) enhanced VEGF mRNA expression. LPS-induced VEGF mRNA accumulation was regulated, at least in part, at the transcriptional level. Enhancement of VEGF gene expression by LPS was shown by gel shift an alysis and use of transcription factor Inhibitors to be mediated via the ac tivation of SP-1.