Enzymatic production of pyruvate from fumarate - an application of microbial cyclic-imide-transforming pathway

For the purpose of producing pyruvate from fumarate through microbial cycli c-imide-transforming pathway, various cyclic-imide-utilizing microorganisms were isolated from soil. Among them, strain g31 was the best producer and was identified as Pseudomonas sp. With the resting cells of the strain, the conditions were optimized for pyruvate production from fumarate. The cells cultivated in the medium containing 2% (w/v) of fumarate showed the highes t production with sufficient yield. The optimized wet-cell concentration, p H and temperature of the reaction were 1% (w/v), pH 6 to 7, and 30 degreesC , respectively. Aeration was found to be an effective factor, and vigorous shaking during the reaction mixture resulted in higher production. Under th e optimized reaction conditions, 100 mM of fumarate was almost stoichiometr ically converted into pyruvate (94 mM) in 24 h. (C) 2001 Elsevier Science B .V. All rights reserved.