Random mutagenesis of Caldariomyces fumago was carried out to produce a chl
oroperoxidase (CPO) with enhanced activity in mixtures of aqueous buffers a
nd organic cosolvents. A mutant CPO with a 3,4-fold increased activity in 4
0% aqueous tert-butyl alcohol was obtained.
A CPO-surfactant conjugate, prepared by colyophilisation, mediated the oxid
ation of thioanisole to its (R)-sulfoxide by TBHP in water-saturated isooct
ane, Copolymerisation of CPO and a toluenediisocyanate prepolymer resulted
in a stable preparation that mediated the oxidation of indole to 2-oxindole
in a range of organic solvents. The highest yield was obtained in 1-octano
l. (C) 2001 Elsevier Science B.V. All rights reserved.