F. Guerard et al., Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacares) wastes using Alcalase, J MOL CAT B, 11(4-6), 2001, pp. 1051-1059
Enzymatic hydrolysis of tuna stomach proteins by Alcalase was investigated
in a batch reactor. The influence of the process variables (enzyme/substrat
e ratio; effect of intermediate substrate and enzyme addition) was studied
with regards to the extent of proteolytic degradation and to the molecular
weight distribution of the peptides. A linear correlation was found between
the degree of hydrolysis (DH) and the enzyme concentration. After addition
of extra substrate during the course of hydrolysis, the final DH obtained
was proportional to the substrate added, suggesting that the concentration
of hydrolysable bonds was one of the main factors controlling the hydrolysi
s rate. Preliminary results showed that tuna protein hydrolysates performed
effectively as nitrogenous source in microbial growth media. (C) 2001 Else
vier Science B.V. All rights reserved.