Purification and characterization of enzymes involved in desulfurization of dibezothiophene in fossil fuels

Authors
Izumi, Y Ohshiro, T
Citation
Y. Izumi et T. Ohshiro, Purification and characterization of enzymes involved in desulfurization of dibezothiophene in fossil fuels, J MOL CAT B, 11(4-6), 2001, pp. 1061-1064
Citations number
8
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN journal
13811177 → ACNP
Volume
11
Issue
4-6
Year of publication
2001
Pages
1061 - 1064
Database
ISI
SICI code
1381-1177(20010122)11:4-6<1061:PACOEI>2.0.ZU;2-I
Abstract
DszC and DszA, dibenzothiophene (DBT) and DBT sulfone monooxygenases, respe ctively, involved in DBT desulfurization, were purified to homogeneity from Rhodococcus erythropolis D-l. The two enzymes were crystallized and enzymo logically characterized. We found a high activity of flavin reductase in th e non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, which is ess ential for DszC and A activities, and purified to homogeneity and character ized the enzyme. (C) 2001 Elsevier Science B.V. All rights reserved.