Mp. Molloy et al., IDENTIFICATION OF WALLABY MILK WHEY PROTEINS SEPARATED BY 2-DIMENSIONAL ELECTROPHORESIS, USING AMINO-ACID-ANALYSIS AND SEQUENCE TAGGING, Electrophoresis, 18(7), 1997, pp. 1073-1078
Micropreparative two-dimensional polyacrylamide gel electrophoresis ha
s been used to separate milk whey proteins from the Tammar wallaby (Ma
cropus eugenii). We have used a combination of amino acid analysis and
N-terminal sequence tagging as a rapid and sensitive method to identi
fy the major whey proteins. Using these techniques, we confidently ide
ntified alpha-lactalbumin and late lactation protein. While these are
the only two M. eugenii whey proteins with a corresponding SWISS-PROT
entry, we demonstrate that by using amino acid analysis and matching a
cross species boundaries, we can identify previously unsequenced conse
rved wallaby whey proteins including beta-lactoglobulin and serum albu
min.