SEPARATION OF DISACCHARIDES AS THEIR BORATE COMPLEXES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT DETECTION IN VISIBLE RANGE

Berate complexation was used to make possible the separation of disacc harides by capillary electrophoresis with indirect detection. A high b erate concentration did not affect the indirect detection sensitivity in as negative a way as predicted previously. The concentration sensit ivity for sucrose was determined to be 2 mM at the berate concentratio n of 200 mM in running electrolyte. The newly introduced backround chr omophore, p-nitrophenol, allows the monitoring of the separation proce ss in a visible range at 400 nm. This also enables the indirect detect ion of W-absorbing compounds in complex mixtures in which they would b e impossible to detect with a W-absorbing backround chromophore.