FROM GENOME TO PROTEOME - PROTEIN MAP OF HAEMOPHILUS-INFLUENZAE

High-resolution two-dimensional (2-D) polyacrylamide gel electrophores is allows the separation of complex biological mixtures (i.e., several hundred proteins from a bacterial cell lysate) in a single experiment . In this report proteins from Haemophilus influenzae were separated b y 2-D gels and analyzed by peptide mass fingerprinting and/or amino ac id analysis. By comparing the peptide mass profiles and the amino acid composition with the Haemophilus influenzae database, 119 protein spa ts were identified. The combination of amino acid analysis and peptide mass fingerprinting is a powerful tool for a rapid and economical ide ntification of a large number of proteins resolved by 2-D gels. Studie s on gene regulation and changes of protein expression upon drug treat ment require quick and serial analysis techniques to efficiently ident ify potential new drug targets.