The objective of this study was to examine the properties of two laser line
s 514 nm and 532 nm when used to image the retina with a scanning laser oph
thalmoscope (SLO), and to compare the images taken with a simultaneous mult
iple wavelength SLO, to those taken with a fundus camera. From this we conc
luded that the 514 nm line is the preferred line for visualising the nerve
fibre layer whereas the 532 nm line is preferred for visualising retinal ve
ssels. Based on these results the 532 nm laser light source was selected as
the green line for imaging with the simultaneous colour SLO. Cases are pre
sented where the colour SLO images contain more information than traditiona
l digitised fundus photographs.