A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector

Authors
Men, AE Meksem, K Kassem, MA Lohar, D Stiller, J Lightfoot, D Gresshoff, PM
Citation
Ae. Men et al., A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector, MOL PL MICR, 14(3), 2001, pp. 422-425
Citations number
22
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN journal
08940282 → ACNP
Volume
14
Issue
3
Year of publication
2001
Pages
422 - 425
Database
ISI
SICI code
0894-0282(200103)14:3<422:ABACLO>2.0.ZU;2-X
Abstract
We constructed a BAC library of the model legume Lotus japonicus with a 6-t o 7-fold genome coverage. We used vector PCLD04541, which allows direct pla nt transformation by BACs. The average insert size is 94 kb. Clones were st able in Escherichia coli and Agrobacterium tumefaciens.