Rapid detection method of beer spoilage bacteria by PCR and universal primers

Authors
Bischoff, E Bohak, I Back, W Leibhard, S
Citation
E. Bischoff et al., Rapid detection method of beer spoilage bacteria by PCR and universal primers, MONATS BRWS, 54(1-2), 2001, pp. 4-8
Citations number
13
Categorie Soggetti
Food Science/Nutrition
Journal title
MONATSSCHRIFT FUR BRAUWISSENSCHAFT
ISSN journal
07231520 → ACNP
Volume
54
Issue
1-2
Year of publication
2001
Pages
4 - 8
Database
ISI
SICI code
0723-1520(200102)54:1-2<4:RDMOBS>2.0.ZU;2-Q
Abstract
A polymerase chain reaction (PCR) for the detection of beer spoilage bacter ia was developed. A pre-enrichment step was done with NBB-C. The PCR was pe rformed with universal primers which amplified the internal transcribed spa cer region between the 16S rDNA and the 23S rDNA. For further identificatio n the PCR-product was digested with restriction endonuclease. This method a ssures a detection of beer spoilage bacteria in the filtrated beer within 4 8 hours.