CLONING OF HUMAN AND RAT P69 CDNA, A CANDIDATE AUTOIMMUNE TARGET IN TYPE-1 DIABETES

Authors
MIYAZAKI I GAEDIGK R HUI MF CHEUNG RK MORKOWSKI J RAJOTTE RV DOSCH HM
Citation
I. Miyazaki et al., CLONING OF HUMAN AND RAT P69 CDNA, A CANDIDATE AUTOIMMUNE TARGET IN TYPE-1 DIABETES, Biochimica et biophysica acta. Molecular basis of disease, 1227(1-2), 1994, pp. 101-104
Citations number
12
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Molecular basis of disease → ACNP
ISSN journal
09254439
Volume
1227
Issue
1-2
Year of publication
1994
Pages
101 - 104
Database
ISI
SICI code
0925-4439(1994)1227:1-2<101:COHARP>2.0.ZU;2-I
Abstract
Triggering of autoimmunity in insulin-dependent diabetes was linked to dietary bovine serum albumin (BSA), Anti-BSA antibodies from diabetes -prone rats precipitate a protein, p69, from islet cell lysates. We ha ve used these antibodies to identify rat p69 cDNAs. Human p69 cDNA was identified by crosshybridization. The p69 coding regions show 87% nuc leotide and 89% amino acid homology. Recombinant p69 is recognized by autoantibody and T cells from diabetic children.