A nude mice model of human rhabdomyosarcoma lung metastases for evaluatingthe role of polysialic acids in the metastatic process

Citation
L. Daniel et al., A nude mice model of human rhabdomyosarcoma lung metastases for evaluatingthe role of polysialic acids in the metastatic process, ONCOGENE, 20(8), 2001, pp. 997-1004
Citations number
42
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ONCOGENE
ISSN journal
09509232 → ACNP
Volume
20
Issue
8
Year of publication
2001
Pages
997 - 1004
Database
ISI
SICI code
0950-9232(20010222)20:8<997:ANMMOH>2.0.ZU;2-8
Abstract
PSA is an oncodevelopmental antigen usually expressed in human tumors with high metastatic potential. Here we set up a metastatic model in nude mice b y using TE671 cells, which strongly express PSA-NCAM. We observed the forma tion of lung metastases when TE671 cells were injected intravenously, intra muscularly, and intraperitoneously, hut not subcutaneously. Intraperitoneou s injections also induced peritoneal carcinosis, ascites, and liver metasta ses. To evaluate the putative role of PSA in the metastatic process we used a specific cleavage of PSA on NCAM by endoneuraminidase-N on intraperitone ous primary tumors. Mice with primary intramuscular tumors were taken as co ntrol. Repeated injections of endoneuraminidase-N led to a decrease in PSA expression in primary intraperitoneous nodules and ascites but not in intra muscular primary tumors. Endoneuraminidase-N also increased the delay in as citic formation and decreased the number of lung or liver metastases in the case of intraperitoneous tumors but not in the case of intramuscular tumor s. When metastases occurred in endoneuraminidase-N injected animals, they s trongly expressed PSA-NCAM. Therefore, we established a relationship betwee n PSA expression on the surface of primary tumor cells and the metastatic p rocess.