Rapid determination of the three-dimensional orientation of single molecules

Authors
Fourkas, JT
Citation
Jt. Fourkas, Rapid determination of the three-dimensional orientation of single molecules, OPTICS LETT, 26(4), 2001, pp. 211-213
Citations number
14
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science","Optics & Acoustics
Journal title
OPTICS LETTERS
ISSN journal
01469592 → ACNP
Volume
26
Issue
4
Year of publication
2001
Pages
211 - 213
Database
ISI
SICI code
0146-9592(20010215)26:4<211:RDOTTO>2.0.ZU;2-M
Abstract
A technique is proposed for determination of the three-dimensional orientat ion of the transition dipoles of single molecules by use of polarization-se nsitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light. (C ) 2001 Optical Society of America OCIS codes: 180.2520, 260.5430.