Effects of the ionic strength and a weak base on the pH profile in chromatofocusing

The formation of segments with increasing pH in descending internal pH prof iles at certain compositions of the starting solution and the eluent was pr edicted theoretically and supported experimentally. Equations for calculati ng the degree of protonation of Lire anion-exchange sorbent as a function o f the parameters of the system were inferred. A combined effect of the ioni c strength of the mobile phase anti the presence of a weak base on the pH p rofile were consider-ed. It was demonstrated that an optimal choice of the ionic strengths of the starting solution and the eluent make it possible to obtain practically linear pH profiles tip to pH 2.0 with the use of st sim ple eluent.