Eh. Hinchcliffe et al., Requirement of a centrosomal activity for cell cycle progression through G(1) into S phase, SCIENCE, 291(5508), 2001, pp. 1547-1550
Centrosomes were microsurgically removed from BSC-1 African green monkey ki
dney cells before the completion of S phase, Karyoplasts (acentrosomal cell
s) entered and completed mitosis, However, postmitotic karyoplasts arrested
before S phase, whereas adjacent control cells divided repeatedly. Postmit
otic karyoplasts assembled a microtubule-organizing center containing gamma
-tubulin and pericentrin, but did not regenerate centrioles. These observa
tions reveal the existence of an activity associated with core centrosomal
structures-distinct from elements of the microtubule-organizing center-that
is required for the somatic cell cycle to progress through G(1) into S pha
se. Once the cell is in S phase, these core structures are not needed for t
he G(2)-M phase transition.