Disruption and functional analysis of six ORFs of chromosome IV: YDL103c (QR11), YDL105w (QR12), YDL112w (TRM3), YDL113c, YDL116w (NUP84) and YDL167c(NRP1)

Authors
Reynaud, A Facca, C Sor, F Faye, G
Citation
A. Reynaud et al., Disruption and functional analysis of six ORFs of chromosome IV: YDL103c (QR11), YDL105w (QR12), YDL112w (TRM3), YDL113c, YDL116w (NUP84) and YDL167c(NRP1), YEAST, 18(3), 2001, pp. 273-282
Citations number
31
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
YEAST
ISSN journal
0749503X → ACNP
Volume
18
Issue
3
Year of publication
2001
Pages
273 - 282
Database
ISI
SICI code
0749-503X(200102)18:3<273:DAFAOS>2.0.ZU;2-5
Abstract
We have disrupted six ORFs (YDL103c, YDL105w, YDL112w, YDL113c, YDL116w and YDL167c) located on the left arm of chromosome IV. Except for YDL112w, the short flanking homology strategy was used to construct disruption cassette s using the KanMX4 marker. For YDL112w, a disruption cassette including the LEU2 gene was made. YDL103c and YDL105w are essential genes for vegetative growth. Disruption of YDL112w, YDL113c and YDL167c does not result in any detectable phenotype with the tests we used, while disruption of YDL116w co nfers slow growth, cryosensitivity and thermosensitivity, and the disrupted diploid homozygotes for the disruption failed to sporulate. Copyright (C) 2001 John Wiley & Sons, Ltd.