Crystallization and preliminary X-ray analysis of a novel pectolytic enzyme, polymethoxygalacturonase SX1 from Trichosporon penicillatum

A novel pectolytic enzyme, polymethoxygalacturonase SX1 from Trichosporon p enicillatum, with a molcular weight of 36 kDa was crystallized by the hangi ng-drop vapour-diffusion method using polyethylene glycol 1000 as a precipi tant. The crystals belonged to the monoclinic space group C2, with unit-cel l parameters a = 165.6, b = 61.0, c = 48.7 Angstrom, beta = 93.1 degrees. T he calculated V-M based on one molecule per asymmetric unit was 3.40 Angstr om (3) Da(-1). A native data set was collected to 2.08 Angstrom resolution from a crystal on a Cu K alpha rotating-anode X-ray source. A molecular-rep lacement solution was obtained using the program AMoRe and the structure of endopolygalacturonase II from Aspergillus niger as a model.