Purpose: To evaluate in vitro the dentin morphology in root canals in terms
of tubule orientation, density and increase in surface area after etching.
Materials and Methods: Thirty anterior teeth were divided in 3 groups at r
andom: the samples of Group 1 were used to study tubular morphology in SEM.
Groups 2 and 3 samples were etched with 32% phosphoric acid. The teeth in
Group 2 were examined by SEM without further treatment. The samples in Grou
p 3 were treated with a bonding system and fiber posts were luted into the
canal. These teeth were then processed for evaluation of hybrid layer forma
tion and resin tags in dentin tubules. The observations were made according
to location in the root dentin, tubule density was estimated, and the incr
ease in area available for bonding after etching was calculated. Results: T
he observations revealed variability in tubule density and orientation with
in different areas of any one sample. Statistically significant differences
in the density of tubules were found depending on location. The dentin sur
face area available for bonding increased by 202% after etching in the cerv
ical third, 156% in the middle third, and 113% in the apical third of the r
oot dentin. Group 3 samples showed that the thickness of the hybrid layer d
epended on the density of tubules. In the sectors with a low density of tub
ules, the hybrid layer was significantly thinner than in areas with a highe
r density of tubules. The increase in dentin surface area might be responsi
ble for the enhanced bond strength after acid etching, but not all areas ex
hibited equal responses to etching.