A novel approach to measuring circulating blood volume: The use of a hemoglobin-based oxygen carrier in a rabbit model

Hemoglobin-based oxygen carriers (HBOC) may be ideal for monitoring circula ting plasma volume (CV-P) and circulating blood volume (CV-B). We used an H BOC (Hemoglobin glutamer-200 [bovine], Oxyglobin(R); Biopure, Cambridge, MA ) as an indicator for relative CV-B in the rabbit model. Accuracy of the te chnique was determined by comparison with the Evans blue dye (EBD) dilution technique in 19 anesthetized female New Zealand rabbits weighing 2.0 to 10 .6 kg. The measurements were per formed at baseline, after hemorrhage (1/3 of CV-B), normovolemic hemodilution (replacement of 1/3 CV-B by Hextend(R); Abbot Laboratories, North Chicago, IL), and hypervolemic hemodilution (add itional infusion of Hextend(R) in a volume equal to 1/3 of CV-B). Hemoglobi n concentration was measured by using a HemoCue(R) photometer (HemoCue AB, Angelholm, Sweden). EBD concentration was analyzed by using linear regressi on to estimate Time 0 concentration; Time 0 was defined as EBD injection ti me. The difference between CV-P values determined by EBD and HBOC dilution was independent from the magnitude of the CV-P value. The relative bias was 1.29 mL and the precision (one SD) was 2.82 mL. The difference did not rea ch statistical significance.