Bacterial PCR in the diagnosis of joint infection

Objectives-To evaluate the value of broad range bacterial FCR in the diagno sis of joint infection and to find out if there are bacteria causing arthri tis which are not cultivable by the present methods. Methods-Polymerase chain reaction (PCR) with broad range bacterial primers and DNA sequencing (bacterial PCR) was used to analyse 154 synovial fluid ( SF) samples from patients with different arthritic diseases. Results-Bacterial DNA was detected in 18 SF samples, including samples from six patients with culture proven purulent arthritis, and from three patien ts with possible purulent arthritis. Three samples from patients with cultu re confirmed purulent arthritis remained negative in bacterial PCR. Conclusions-The results indicate that in the usual diagnostic laboratory se tting bacterial PCR does not offer any obvious advantage over bacterial cul ture in the microbiological diagnosis of joint infection.