Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer

Authors
Viera, CT Tsiodras, S Gold, HS Coakley, EPG Wennersten, C Eliopoulos, GM Moellering, RC Inouye, RT
Citation
Ct. Viera et al., Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer, ANTIM AG CH, 45(3), 2001, pp. 973-975
Citations number
22
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
45
Issue
3
Year of publication
2001
Pages
973 - 975
Database
ISI
SICI code
0066-4804(200103)45:3<973:ROVSIE>2.0.ZU;2-9
Abstract
We assessed the ability of gene transfer to reverse vancomycin resistance i n class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette ful ly restored vancomycin susceptibility through a combined transcriptional ac tivator binding domain decoy and inducible vanA antisense RNA effect.