Characterization of a divergent vanD-type resistance element from the first glycopeptide-resistant strain of Enterococcus faecium isolated in Brazil

Authors
Dalla Costa, LM Reynolds, PE Souza, HAPHM Souza, DC Palepou, MFI Woodford, N
Citation
Lm. Dalla Costa et al., Characterization of a divergent vanD-type resistance element from the first glycopeptide-resistant strain of Enterococcus faecium isolated in Brazil, ANTIM AG CH, 44(12), 2000, pp. 3444-3446
Citations number
20
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
44
Issue
12
Year of publication
2000
Pages
3444 - 3446
Database
ISI
SICI code
0066-4804(200012)44:12<3444:COADVR>2.0.ZU;2-5
Abstract
Enterococcus faecium 10/96A from Brazil was resistant to vancomycin (MIC, 2 56 mug/ml) but gave no amplification products with primers specific for kno wn van genotypes. A 2,368-bp fragment of a van cluster contained one open r eading frame encoding a peptide with 83% amino acid identity to VanH(D), an d a second encoding a D-alanine-D-lactate ligase with 83 to 85% identity to VanD. The divergent glycopeptide resistance phenotype was designated VanD4 .