Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum

Authors
Durand, R Eslahpazire, J Jafari, S Delabre, JF Marmorat-Khuong, A di Piazza, JP Le Bras, J
Citation
R. Durand et al., Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum, ANTIM AG CH, 44(12), 2000, pp. 3461-3464
Citations number
25
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
44
Issue
12
Year of publication
2000
Pages
3461 - 3464
Database
ISI
SICI code
0066-4804(200012)44:12<3461:UOMBTD>2.0.ZU;2-M
Abstract
PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point m utation in Plasmodium falciparum. One hundred African clinical isolates wer e tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a pr omising tool for epidemiological studies.