Js. Ryu et al., NMDA receptor and NO mediate ET-1-induced behavioral and cardiovascular effects in periaqueductal gray matter of rats, ARCH PH RES, 24(1), 2001, pp. 64-68
Endothelin-1 (ET-1), a novel and potent vasoconstrictor in blood vessel, is
known to have some functions in the rat central nervous system (CNS). In o
rder to investigate the central functions of ET-1, ET-1 was administered to
the periaqueductal gray area (PAG) of anesthetized rats to induce barrel r
olling and increase the arterial blood pressure (ABP). ET-1 had a modulator
y effect on central cardiovascular and behavioral control. The selective N-
methyl-D-aspartate (NMDA) receptor antagonist MK-801 (3 mu mol/kg, i.p.) bl
ocked the ET-1 induced responses, and both the nitric oxide synthase (NOS)
inhibitor L-NAME (N-nitro-L-arginine methylester 1 mmol/rat) and the nitric
oxide (NO) scavenger hemoglobin (15 nmol/rat) had similar effects in reduc
ing the ET-1 (10 pmol/rat)-induced behavioral changes and ABP elevation. Ho
wever, NO donor sodium nitroprusside (SNP 10 mug, 1 mug/rat) decreased the
ET-1 induced ABP elevation, and recovered the ET-1-induced barrel rolling e
ffect that was reduced by MK-801. These results suggest that ET-1 might hav
e neuromodulatory functions such as ABP elevation and barrel rolling induct
ion in the PAG of the rats via the NMDA receptor and NO.