Aa. Paul et al., Inhibition of HERG potassium channel current by the Class 1a antiarrhythmic agent disopyramide, BIOC BIOP R, 280(5), 2001, pp. 1243-1250
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The Class 1a antiarrhythmic drug disopyramide (DISO) is associated with 'ac
quired' prolongation of the QT interval of the electrocardiogram (ECG). Thi
s potentially proarrhythmic effect is likely to reflect drug actions on ion
channels involved in ventricular action potential repolarisation. In this
study, we examined the effects of DISO on potassium channels encoded by HER
G, as this K channel type has been implicated in both congenital and acquir
ed long-QT syndromes (LQTS). Chinese hamster ovary cells were transiently t
ransfected with HERG cDNA for subsequent whole cell patch clamp recording.
HERG tail currents recorded at -40 mV following test pulses to +30 mV were
inhibited in a dose-dependent fashion by DISO concentrations within the cli
nical range (IC50 = 7.23 +/- 0.72 muM; mean +/- SEM), Experiments with 10 m
uM DISO indicated that the degree of HERG blockade showed some voltage depe
ndence. Further data obtained using an 'envelope of tails' protocol (pulse
potential +40 mV) were consistent with a significant role for open-channel
blockade at lower drug concentrations. At higher concentrations it is possi
ble that blockade may have involved drug binding to both resting and open c
hannels. Inhibition of the inactivation-deficient mutant HERG-S631A was com
parable to that seen for wild-type HERG. Therefore, channel inactivation wa
s not obligatory for DISO to exert its effect. Native delayed rectifier tai
l currents from rabbit isolated ventricular myocytes were also inhibited by
DISO, We conclude (a) that DISO inhibits HERG encoded potassium channels a
t clinically relevant concentrations and (b) that this action may constitut
e the molecular basis for acquired LQTS associated with this drug. (C) 2001
Academic Press.