Structural organization of the mouse phosphatidylinositol 3-kinase p110d gene

Phosphatidylinositol 3-kinases are a family of dual specificity lipid/prote in kinases, The products of PI3K's, phosphatidylinositol (3,4,5) triphospha te and phosphatidylinositol(3,4) bisphosphate, act as second messengers con necting activated transmembrane receptors to signaling pathways that contro l gene transcription, proliferation, transformation, programmed cell death, adhesion, migration and vesicular transport. There is evidence that differ ent isoforms of PI3K's activate specific signaling pathways and are thus re sponsible for integrating cellular responses. The elucidation of the genomi c structure of the catalytic subunits is a necessary step for the investiga tion of the function of PI3K isoforms by inactivation of the gene in vivo. The structural organization of p110 alpha, beta, and gamma genes has been p reviously reported. Here we report the cloning, sequencing, and structural organization of the mouse p110 delta gene from a murine 129/Sv genomic libr ary. The p110 delta gene consists of 22 exons and spans over 13 kb, Compari son of the genomic structure with that of p110 alpha, beta, and gamma demon strates that the p110 delta gene shares its exon structure with p110 beta, the most closely related PI3K at the amino acid level. (C) 2001 Academic Pr ess.