Desensitization of neuronal nicotinic acetylcholine receptors conferred byN-terminal segments of the beta 2 subunit

Desensitization is a general property of ligand-gated ion channels. Because of a wide array of available subunit combinations, it generates different time constants for channel closure, thereby modulating the processing of in formation in the brain. Within the family of neuronal nicotinic acetylcholi ne receptors (nAChRs), alpha3 beta2 and alpha3 beta4 receptors display cont rasting properties of desensitization. When measured using two-electrode vo ltage-clamp in Xenopus oocytes, desensitization results in current decrease s 2 s after initiation of acetylcholine application by 94% for alpha3 beta2 receptors, but only by 6% in the case of alpha3 beta4 receptors. Desensiti zation was analyzed by inserting different portions of the beta2 into the b eta4 subunit. Residues 1-212 of the beta2 subunit were able to confer 78% d esensitization in 2 s, while smaller chimeras revealed desensitization in 2 s conferred by residues 1-42 alone to a level of 50%, by residues 72-89 to a level of 74%, and by residues 96-212 to a level of 77%. Some long-term ( 25 min) effects of desensitization driven by acetylcholine were found to re ly partially on the same elements, including an enhancement mediated by res idues 1-95 and 96-212 of the beta2 subunit individually. Our results reveal that desensitization relies independently on diverse portions of the extra cellular domain of the beta2 subunit. Phenotype of alpha3 beta4 involves, i n contrast, complex structural requirements involving residues dispersed th roughout the entire N-terminal domain of the beta4 subunit.