D. Mohri et al., Change in gene expression in facial nerve nuclei and the effect of superoxide dismutase in a rat model of ischemic facial paralysis, BRAIN RES, 893(1-2), 2001, pp. 227-236
Peripheral nerve injury induces changes in gene expressions of a variety of
neuroactive substances: in cell somata, which may have roles in the adapti
ve response to the injury, neuronal survival, growth and regeneration. In t
his study, we designed a rat model of ischemic peripheral facial paralysis
with a selective embolization technique, and observed mRNA expression of ca
lcitonin gene-related peptide (CGRP), c-jun, and growth associated protein
(GAP)-43 in facial nerve nuclei using in situ hybridization histochemistry.
The rats were demonstrated to have a transient facial paralysis consistent
ly, and thus this method was regarded as a model of minor peripheral nerve
injury. The mRNA of CGRP, c-jun and GAP-43 showed a distinct pattern of ind
uction and time course of increase after the ischemic nerve injury. The res
ults suggest that the small injury to the peripheral nerve was able to indu
ce changes in mRNA expression in the cell body of motoneurons. We also inve
stigated the protective effect of superoxide dismutase (SOD), which is a fr
ee radical-scavenging enzyme involved in cellular antioxidant defenses. The
SOD treatment clearly alleviated the behavioral impairment and decreased t
he CGRP mRNA expression at 3rd day after injury. These data suggest that a
free radical generated by the ischemia may be partially responsible for isc
hemic nerve damage and the change in gene expression in motoneurons. (C) 20
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