Investigating the function of Akt by tet-off inducible expression system

A tet-off inducible cell line named BBT derived from BA/F3 beta cell line w as constructed and the effect of this inducible expression system was signi ficant when detected by tet-off responded luciferase reporter gene assay. T hen tet-off responded Akt expression plasmid was transfected into BET cells , and the stable cell lines were screened. The result of Northern blot show ed that the expression of akt was significantly inducible. The clone with t he best inducible effect was selected and named BBA for investigating the f unction of Akt. We found that Akt could significantly inhibit zinc-induced decrease of cell viability when assayed by MTT method. And the flow cytomet ric analysis showed that Akt could markedly repress zinc-induced apoptosis.