Phenotypic and phylogenetic characterization of Burkholderia (Pseudomonas)sp strain LB400

The relevant phenotypic traits and phylogenetic relationships between Burkh olderia (Pseudomonas) sp, strain LB400 and B. cepacia ATCC 25416(T) were co mpared to determine the degree to which these two strains might be related. Strain LB400 degrades chlorinated biphenyls and has been a model system fo r potential use in the bioremediation of polychlorinated biphenyls, while s ome strains of B. cepacia are plant and human pathogens. The fatty acid met hyl ester profile, sole carbon source utilization, and biochemical tests co nfirmed that strain LB400 was a member of the genus Burkholderia. The 16S r RNA gene sequence showed that this strain was not as closely related to B. cepacia as previously suspected or to other known pathogens of this genus, but is closely related to B. phenazinium, B. caribensis, B, graminis, and t hree unnamed Burkholderia spp. not known to be pathogenic.