Functional properties of circulating and transmigrated neutrophils in a rat peritonitis model

Using a rat peritonitis model, we examined the pattern of expression of adh esion molecules and H2O2 productivity of neutrophils by indirect immunofluo rescence and flow cytometry. H2O2 production in blood neutrophils was signi ficantly increased after peritonitis induction. The expression of CD11b and CD18 antigens on blood neutrophils was also significantly increased after peritonitis induction. In contrast, H2O2 production in peritoneal neutrophi ls was significantly lower than that in blood neutrophils. The expression o f CD11b and CD45 antigens on peritoneal neutrophils was significantly highe r than on blood neutrophils. Although no function has been assigned to LFA- 1 on neutrophils, the expression of LFA-1 (CD11a/CD18) on peritoneal neutro phils was significantly decreased. Copyright (C) 2001 S. Karger AG, Basel