Use of an immunoassay as a means to detect polychlorinated biphenyls in animal fat

Citation
S. Jaborek-hugo et al., Use of an immunoassay as a means to detect polychlorinated biphenyls in animal fat, FOOD ADDIT, 18(2), 2001, pp. 121-127
Citations number
22
Categorie Soggetti
Food Science/Nutrition
Journal title
FOOD ADDITIVES AND CONTAMINANTS
ISSN journal
0265203X → ACNP
Volume
18
Issue
2
Year of publication
2001
Pages
121 - 127
Database
ISI
SICI code
0265-203X(200102)18:2<121:UOAIAA>2.0.ZU;2-B
Abstract
In this paper we describe the validation and utilization of the Hybrizyme(T M) DELFIA(R) immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimized to detect concentrations of PCBs in animal fat at or above 200 ng/g. The limit of detection of the assay was less than 50 ng/ g. When the action level for the DELFIA assay was set at 100 ng/g to detect samples having 200 ng/g or more PCBs, the false negative rate was estimate d at 0.2%. The false positive rate depends on the concentration of PCBs in the sample population and was estimated at 4% and 7% for samples having 10 ng/g and 20 ng/g PCBs, respectively. By utilizing the DELFIA assay system t o select for positive samples and GC/MS analysis for confirmation analysis, an accurate and cost-effective programme has been established for the high throughput detection of PCBs in animal products.