Hydroxymethylfurfural determination in infant milk-based formulas by micellar electrokinetic capillary chromatography

A procedure for determining hydroxymethylfurfural (HMF) in milk-based produ cts by micellar electrokinetic capillary chromatography (MECC) was settled on. The effect of the trichloroacetic acid on the migration time of HMF and presence of interference peaks were investigated. MECC procedure was appli ed on commercial liquid infant formulas and compared with the classical rev ersed-phase high performance liquid chromatography method, giving similar v alues of repeatability and recovery. HMF peak was well-resolved by using an uncoated fused-silica capillary (48.5 cmx50 mum i.d.) with 50 mM phosphate buffer (pH 7.5) containing 100 mM SDS as electrolyte, voltage at 20 kV (25 degreesC). sample injection was by pressure (50 mbar, hydrodynamic) for 2. 5 s. HMF analysis by MECC was suitable for routine analysis since separatio n was completely achieved at 5 min. (C) 2001 Elsevier Science Ltd. All righ ts reserved.