Identification of clathrin-adaptor medium chains in Dictyostelium discoideum: differential expression during development

Clathrin-adaptor complexes (APs) are vesicle coat components that participa te in cargo selectivity;ind transport vesicle formation. Here we cloned and characterized apm1, apm3 and apm4 cDNAs encoding AP medium chains (mu) in D. discoideum. Amino acid comparison suggested that predicted proteins were homologous to known mu1, mu3 and mu4 subunits of mammalian APs as they sha red 69, 51, and 26% identity with mouse mu 1A, human mu 3A and human mu4, r espectively. In all chains, amino acid residues predicted to interact with tyrosine based sorting signals were conserved. Southern blot analysis indic ated only one copy of each gene in D. discoideum genome. Expression of apm1 and apm3 mRNAs stayed relatively constant duping vegetative growth and thr oughout development. In contrast. apm4 was poorly expressed in amoebae but became well detectable by RT-PCR upon cell differentiation. This regulated expression of coat proteins enlightens the importance of intracellular memb rane transport vesicles during development in D. discoideum and strengthens this attractive model organism for studying the function of coat complexes in vivo. (C) 2001 Elsevier Science B.V. All rights reserved.