In this review we identify the elemental signals that regulate eosinophil a
ccumulation in the allergic lung. We show that there are two interwoven mec
hanisms for the accumulation of eosinophils in pulmonary tissues and that t
hese mechanisms are linked to the development of airways hyperreactivity (A
HR). Interleukin-(IL)-5 plays a critical role in the expansion of eosinophi
l pools in both the bone marrow and blood in response to allergen provocati
on of the airways. Secondly. IL-4 and IL-13 operate within the allergic lun
g to control the transmigration of eosinophils across the vascular bed into
pulmonary tissues. This process exclusively promotes tissue accumulation o
f eosinophils. IL-13 and IL-4 probably act by activating eosinophil-specifi
c adhesion pathways and by regulating the production of IL-5 and eotaxin in
the lung compartment. IL-5 and eotaxin co-operate locally in pulmonary tis
sues to selectively and synergistically promote eosinophilia. Thus, IL-5 ac
ts systemically to induce eosinophilia and within tissues to promote local
chemotactic signals. Regulation of IL-5 and eotaxin levels within the lung
by IL-4 and IL-13 allows Th2 cells to elegantly co-ordinate tissue and peri
pheral eosinophilia. Whilst the inhibition of either the IL-4/IL-13 or IL-5
/eotaxin pathways resulted in the abolition of tissue eosinophils and AKR,
only depletion of IL-5 and eotaxin concurrently results in marked attenuati
on of pulmonary inflammation. These data highlight the importance of target
ing both IL-5 and CCR3 signalling systems for the resolution of inflammatio
n and AHR associated with asthma.