Immunoaffinity sample purification and MALDI-TOF MS analysis of alpha-solanine and alpha-chaconine in serum

A sample purification technique was developed for the detection of potato g lycoalkaloids (GAs) in blood serum by matrix-assisted laser desorption/ioni zation time-of-flight mass spectrometry (MALDI-TOF MS). GAs were extracted from spiked serum (5 mL) using a C-18 solid-phase extraction cartridge. The GAs were then selectively captured an antibody-coated agarose beads. The a garose beads were washed with water and the GAs eluted with 25 muL of metha nol. MALDI-TOF MS was used to detect the GAs in the methanol eluent. Immuno affinity sample purification df the GAs effectively reduced the signal Supp ression observed during the analysis of unpurified samples. alpha -Chaconin e and alpha -solanine were detected in serum spiked with 1 ng/mL of each GA .