Supercritical fluid extraction of 5-hydroxymethyl-2-furaldehyde from raisins

An extraction method based on supercritical CO2 has been developed for the analysis of 5-hydroxymethyl-2-furaldehyde in raisins. To optimize extractio n variables, a fractional factorial experimental design was applied. Six ex traction variables were optimized. The organic modifier used for increasing the extraction fluid solvating power was the most important factor. Methan ol as organic modifier produced 10-fold higher recoveries of 5-hydroxymethy l-2-furaldehyde than ethyl acetate. The efficiency of the organic modifier in the static extraction phase was compared with using it in the dynamic ex traction phase. Repeatability of the analysis method was evaluated, which r esulted in an RSD of <5%. 5-Hydroxymethyl-2-furaldehyde was quantified in r aisins, and the concentration was found to be 0.128 mg/g of raisin.