New approach for accurate simulation of human pharmacokinetics in an in vitro pharmacodynamic model: application to ciprofloxacin

An in vitro pharmacodynamic model using a disposable dialyser unit and comp uter-controlled devices was developed. Feedback control of peristaltic pump flow rates is used to maintain constant flow rates, thus avoiding the prob lem of the modification of the physical properties of the tubing that gener ally occurs. Fast equilibrium is obtained with capillaries, which allows si mulation of the same kinetic profile in the central and the peripheral comp artments. Thus, more accurate simulation of plasma, extracapillary fluid or whole tissue kinetics can be performed. Our model was validated by simulat ion of a 30 min infusion of a 200 mg dose, and of an oral administration of a 500 mg dose of ciprofloxacin.