Bioactive chemical constituents from Alchornea laxiflora (benth) pax and hoffman

Quercetin-7,4'-disulphate (1) quercetin (2) quercetin-3',4'-disuIphate (3) quercetin-3,4'-diacetate (4) rutin (5) and quercetrin (6) were isolated fro m the ethyl acetate soluble fraction (ESM) of the crude methanolic leaf ext ract of Alchornea laxiflora. Purification of these compounds was carried ou t by column chromatography utilising sephadex LH 20 and various mixtures of water, methanol, ethanol and toluene as eluents. Structural elucidation wa s by UV, IR,(HNMR)-H-1 and (CNMR)-C-13 spectroscopy as well as by FAB-MS. A ntimicrobial activity of isolated compounds was detected in Gram-positive, Gram-negative and fungal organisms. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.