Y chromosome compound haplotypes with the microsatellite markers DXYS265, DXYDS266, and DXYS241

Two newly developed microsatellite markers on Yp11 (DXYS265) and Yq11.21 (D XYS266) and our previously reported marker, on Yp11 (DXYS241), were typed b y tripler polymerase chain reaction (PCR) in 102 Japanese, 18 white America n, and 17 black American males. The DXYS265 locus revealed three alleles, t he DXYS266 locus showed two alleles, while the DXYS241 locus showed five al leles. Nine different compound haplotypes were observed among the males. Of these, two haplotypes were common to an groups, while four were limited to Japanese. Pedigree analysis of 61 Japanese families revealed no mutations of these loci. The tripler PCR developed in this study, as well as the new loci: are useful for tracing paternal Lineages in human migration studies a nd population analysis, in addition to Y chromosome evolutionary studies.