The effect of cytokines on the migration of fibroblasts derived from different regions of the canine shoulder capsule

This study examined the effect of several cytokines on the chemotactic migr ation of fibroblasts derived from 3 different pan's of the canine shoulder: the upper part of the medial glenohumeral ligament (equivalent to the ante rior part of the inferior glenohumeral ligament of the human shoulder); the inferior part of the medial glenohumeral ligament (equivalent to the axill ary pouch of the human shoulder); and the posterior capsule (equivalent to the thin poster;or capsule in the human shoulder). Platelet-derived growth factor-AB stimulated the migration of all 3 cell types in a dose-dependent manner, with increases From 150% to 300% at 1 ng/mL to 500% to 700% at 70 n g/ml. Hepatocyte growth factor also stimulated the migration of all 3 cell types in a dose-dependent manner (130% to 310%). Insulinlike growth factor- 1 increased the migration of all 3 types of fibroblasts by 160% to 250%. Bo ne morphogenic protein-2 interleukin-1, and transforming growth factor-beta had no significant effect on migration of shoulder capsular fibroblasts. T hese data demonstrate that capsular fibroblasts are responsive to specific growth factors and suggest the potential for use of growth factors to augme nt healing and/or remodeling of the shoulder capsule.