PHYLOGENETIC-RELATIONSHIPS IN THE PAPILIONOIDEAE (FAMILY LEGUMINOSAE)BASED ON NUCLEOTIDE-SEQUENCES OF CPDNA (RBCL) AND NCDNA (ITS-1 AND ITS-2)

Sequences of cpDNA (rbcL) were determined for 94 species and of ncDNA [ITS 1+2 regions (internal transcribed spacer) of rDNA] for 75 species representing mainly the papilionoid tribes Sophoreae, Thermopsideae, Podalyrieae, Liparieae, Crotalarieae, and Genisteae. Sequence data wer e used to reconstruct the underlying molecular phylogeny. Several clus ters and furcations were identical in the rbcL and ITS trees of the Pa pilionoideae, indicating that a reticulate evolution due to past hybri dization of members from different tribes and genera is unlikely: The Sophoreae (especially Styphnolobium japonicum (syn. Sophora japonica) and Sophora secundiflora) are positioned at the base of the papilionoi d tree, whereas some other Sophora species (Sophora davidii, flavescen s, jaubertii, microphylla) are closely related to Thermopsideae/Podaly rieae, The Thermopsideae/Podylyrieae cluster (including Liparieae) sha res ancestry with the Crotalarieae and Genisteae. Argyrolobium (Africa n taxa) and Melolobium cluster between Crotalarieae and Genisteae. In the Genisteae three clusters are apparent: the monophyletic genus Lupi nus, the Cytisus-, and the Genista-group. According to this analysis, the Cytisus-complex includes Cytisus, Lembotropis, Chamaecytisus, Spar tocytisus, and Calicotome. The Genista-group consists of Genista, Teli ne, and Chamaespartium sagittale. Other genera (e.g., Adenocarpus, Arg yrocytisus, Cytisophyllum, Erinacea, Laburnum, Petteria, Retama, Spart ium, and Ulex) could not be attributed unequivocally to the Cytisus or Genista complex. (C) 1997 Academic Press.