L. Teberikler et al., Selective extraction of phosphatidylcholine from lecithin by supercriticalcarbon dioxide/ethanol mixture, J AM OIL CH, 78(2), 2001, pp. 115-119
Selective extraction of phosphatidylcholine (PC) from deoiled soybean lecit
hin using supercritical fluid (SCF) mixtures of carbon dioxide (CO2) and et
hanol was studied at moderate pressures. Temperature was varied between 60
and 80 degreesC at pressures of 17.2 and 20.7 MPa. Ethanol was added as co-
solvent to supercritical CO2 at the levels of 10 and 12.5 wt%. Constant rat
e of extraction of the individual phospholipids (PL) was observed for 150 m
in during which the extractions were carried out. Pressure and ethanol frac
tion had a positive effect on the selective extraction of PC, whereas tempe
rature had a negative effect. Under all the conditions studied, the extract
s were mainly composed of PC while the extraction of the other PL was very
low. Extraction at 60 degreesC and 20.7 MPa with 10 wt% ethanol/90 wt% CO2
SCF mixture resulted in 95% selectivity to PC.