Occurrence and virulence factors of non-O157 Shiga toxin-producing Escherichia coli in retail meat in Dunedin, New Zealand

Authors
Brooks, HJL Mollison, BD Bettelheim, KA Matejka, K Paterson, KA Wark, VK
Citation
Hjl. Brooks et al., Occurrence and virulence factors of non-O157 Shiga toxin-producing Escherichia coli in retail meat in Dunedin, New Zealand, LETT APPL M, 32(2), 2001, pp. 118-122
Citations number
30
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
02668254 → ACNP
Volume
32
Issue
2
Year of publication
2001
Pages
118 - 122
Database
ISI
SICI code
0266-8254(200102)32:2<118:OAVFON>2.0.ZU;2-R
Abstract
Retail raw meat was sampled for the presence of Shiga toxin-producing Esche richia coli (STEC) using enrichment culture and Vero cell assay. The STEC o btained were serotyped and tested for enterohaemolysin (Ehly) production an d the eae gene. The presence of Shiga toxin genes (stx) was confirmed by po lymerase chain reaction. A total of 18 STEC were isolated accounting for 12 % of beef, 17% of lamb and 4% of pork samples. Five isolates produced Ehly but none possessed the eae gene. Five isolates were identified which posses sed the stx(2) gene and belonged to serotypes associated with severe infect ion.