EFFECT OF CYSTEINESULFINATE ON FATTY ACID-DEPENDENT UNCOUPLING - MODULATION OF RECOUPLING BY SUBSTRATES OF THE ASPARTATE GLUTAMATE ANTIPORTER AND DIETHYL PYROCARBONATE/

The action of cysteinesulfinate, the substrate of the aspartate/glutam ate antiporter on the palmitate-induced uncoupling in rat liver mitoch ondria and the recoupling effect of glutamate, aspartate, and diethyl pyrocarbonate was studied. In the presence of palmitate and an inhibit or of the ADP/ATP-antiporter carboxyatractylate, cysteinesulfinate exe rted a relatively week recoupling effect. However, it significantly de creased the recoupling action of glutamate, aspartate, and diethyl pyr ocarbonate. In the presence of cysteinesulfinate, these compounds caus ed recoupling at higher concentrations, The data show that the recoupl ing action of glutamate, aspartate, and diethyl pyrocarbonate is due t o their interaction with the aspartate/glutamate antiporter. The data also confirm the suggestion that this anion carrier is involved in the uncoupling action of fatty acids.