STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROVIDENCIA-ALCALIFACIENS-O23 CONTAINING A NOVEL COMPONENT - AN AMIDE OF D-GLUCURONIC ACID WITH N-EPSILON-(1-CARBOXYETHYL)LYSINE

An acidic O-specific polysaccharide was obtained by mild acid degradat ion of the lipopolysaccharide of the bacterium Providencia alcalifacie ns O23 and found to contain D-glucose, D-galactose, 2-acetamido-2-deox y-D-galactose, and on-(1-carboxyethyl)-N-alpha-(D-glucuronoyl)lysine. On the basis of full and partial acid hydrolyses, selective solvolysis with anhydrous hydrogen fluoride, and H-1- and C-13-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear H-1, C-13 multi-quantum coherence (HMQC), and rotating- frame nuclear Overhauser effect spectroscopy (ROESY), the following st ructure of the liner tetrasaccharide repeating unit of the polysacchar ide was established: [GRAPHICS] CS]